Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33]

Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33]

• Description: GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel β-sheet, a parallel β-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with γ-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from γ-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline.
• Product Name Alternative: epididymis secretory sperm binding protein Li 64p antibody epididymis secretory sperm binding protein Li 88n antibody Glutathione synthase antibody Glutathione synthetase antibody GSH S antibody GSH synthetase antibody GSH-S antibody GSHB_HUMAN antibody GSHS antibody GSS antibody HEL-S-64p antibody HEL-S-88n antibody MGC14098 antibody OTTHUMP00000030711 antibody
• Abbreviation: GSH synthetase, GSH-S, GSS
• CAS Number: 9000-83-3
• Swiss Prot: P48637 Human, P51855 Mouse, P46413 Rat
• Cellular Locus: Cytosol. Extracellular region or secreted.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within Human Glutathione Synthetase aa 301-420 / 474.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC
• Positive Control: SiHa cell lysate, rat kidney tissue lysate, mouse colon tissue lysate, rat liver tissue lysate, LOVO, human tonsil tissue, human colon carcinoma tissue, human kidney tissue, rat epididymis tissue, mouse colon tissue, HepG2.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 52 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes