ZAP70 Recombinant Rabbit Monoclonal Antibody [JU08-39]

ZAP70 Recombinant Rabbit Monoclonal Antibody [JU08-39]

• Description: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.
• Product Name Alternative: 70 kDa zeta associated protein antibody 70 kDa zeta-associated protein antibody EC 2.7.10.2 antibody FLJ17670 antibody FLJ17679 antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Syk-related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody Tyrosine-protein kinase ZAP-70 antibody TZK antibody ZAP 70 antibody ZAP70 antibody ZAP70_HUMAN antibody Zeta chain associated protein kinase 70kD antibody Zeta chain associated protein kinase 70kDa antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody Zeta chain of T cell receptor associated protein kinase 70 antibody Zeta chain TCR associated protein kinase 70kD antibody Zeta chain TCR associated protein kinase 70kDa antibody
• Abbreviation: ZAP70, SRK
• CAS Number: 9000-83-3
• Swiss Prot: P43403 Human
• Cellular Locus: Cytoplasm. Cell membrane.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within Human ZAP70 aa 570-619 / 619.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC, IP
• Positive Control: Human thymus tissue lysates, Jurkat cell lysates, LOVO, SH-SY-5Y, human tonsil tissue, human spleen tissue, 商品名 was done on Jurkat.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 70 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes