ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36]

• Description:

  Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

• Product Name Alternative:

  ATG 1 antibody ATG1 antibody ATG1 autophagy related 1 homolog antibody ATG1A antibody Autophagy related protein 1 homolog antibody Autophagy-related protein 1 homolog antibody FLJ38455 antibody FLJ46475 antibody hATG1 antibody KIAA0722 antibody Serine/threonine protein kinase ULK1 antibody Serine/threonine protein kinase Unc51.1 antibody Serine/threonine-protein kinase ULK1 antibody ULK 1 antibody ULK1 antibody ULK1_HUMAN antibody Unc 51 (C. elegans) like kinase 1 antibody UNC 51 antibody Unc 51 like kinase 1 antibody Unc-51 like kinase 1 (C. elegans) antibody Unc-51-like kinase 1 antibody UNC51 antibody UNC51, C. elegans, homolog of antibody Unc51.1 antibody

• Abbreviation:

  ATG1, hATG1, ULK1, KIAA0722

• Swiss Prot:

  O75385 Human, O70405 Mouse, D3ZMG0 Rat

• Cellular Locus:

  Cytosol, Preautophagosomal structure.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within Human ULK1 aa 930-979 / 1050.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P

• Positive Control:

  A-172 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, MCF7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, human spleen tissue, rat skeletal muscle tissue, human colon carcinoma tissue, mouse brain tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:5,000 ;IHC-P: 1:50-1:200

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 113 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3