ULK1 Recombinant Rabbit mAb

• Background:

  Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

• Structure Composition:

  Store at -20°C. Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

• Product Name Alternative:

  ATG1; ATG1A; UNC51; hATG1; Unc51.1

• Swiss Prot:

  O75385

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide of human ULK1

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF

• Purity:

  Affinity Purification

• Modification:

  Unmodification

• Molecular Weight:

  130 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of extracts from PC-3 cells using it at 1:1000.

• Host or Source:

  Rabbit

• CAS Number:

  9000-83-3