14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ084-3]

14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ084-3]

• Description: The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.
• Product Name Alternative: 14-3-3 protein beta/alpha Protein 1054 Protein kinase C inhibitor protein 1 KCIP-1 14-3-3 protein beta/alpha, N-terminally processed YWHAB 14-3-3 protein zeta/delta YWHAZ
• Abbreviation: KCIP-1, YWHAB, YWHAZ
• CAS Number: 9000-83-3
• Swiss Prot: P31946 Human, P63104 Human, P63101 Mouse, Q9CQV8 Mouse, P35213 Rat, P63102 Rat
• Cellular Locus: Cytoplasm, Melanosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within Human 14-3-3 aa 1-122 / 246.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: NIH/3T3 cell lysate, SK-Br-3 cell lysate, human skin tissue lysates.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:5,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 28 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes