Topoisomerase I Recombinant Rabbit Monoclonal Antibody [SC69-03]

Topoisomerase I Recombinant Rabbit Monoclonal Antibody [SC69-03]

• Description: DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.
• Product Name Alternative: DNA topoisomerase 1 antibody DNA topoisomerase I antibody NUP98 fusion gene antibody TOP 1 antibody TOP I antibody TOP1 antibody TOP1_HUMAN antibody TOPI antibody Topoisomerase (DNA) I antibody Topoisomerase 1 antibody Topoisomerase1 antibody TopoisomeraseI antibody Type I DNA topoisomerase antibody
• Abbreviation: TOP1
• CAS Number: 9000-83-3
• Swiss Prot: P11387 Human, Q04750 Mouse, Q9WUL0 Rat
• Cellular Locus: Nucleus, nucleoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human TOP1 aa 1-49 / 765.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
• Positive Control: MCF7 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, MCF7, Hela, SHG-44, 293, HepG2, mouse brain tissue, human large intestine tissue, mouse hippocampus tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:20,000 ;IF-Cell: 1:50-1:1,000 ;IF-Tissue: 1:50-1:500 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 91 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes