Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]

• Description: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
• Product Name Alternative: C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog (S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog (S. pombe) antibody CHK1_HUMAN antibody EC 2.7.11.1 antibody rad27 antibody Serine/threonine protein kinase Chk1 antibody Serine/threonine-protein kinase CHK1 antibody STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
• Abbreviation: CHEK1, CHK1
• CAS Number: 9000-83-3
• Swiss Prot: O14757 Human, O35280 Mouse
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within Human Chk1 aa 431-476 / 476.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell
• Positive Control: Hela cell lysate, Daudi cell lysate, HL-60 cell lysate, HeLa, MCF-7, PC-3M.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 54 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes