Phospho-p53 (S33) Recombinant Rabbit Monoclonal Antibody [JE41-97]

Phospho-p53 (S33) Recombinant Rabbit Monoclonal Antibody [JE41-97]

• Description: p53, also known as Tumor protein P53, cellular tumor antigen p53 (UniProt name), or transformation-related protein 53 (TRP53) is a regulatory protein that is often mutated in human cancers. The p53 proteins (originally thought to be, and often spoken of as, a single protein) are crucial in vertebrates, where they prevent cancer formation. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene. The TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation. TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome. In addition to the full-length protein, the human TP53 gene encodes at least 12 protein isoforms.
• Product Name Alternative: Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody tumor antigen p55 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody
• Abbreviation: TP53, P53
• CAS Number: 9000-83-3
• Swiss Prot: P04637 Human, P02340 Mouse, P10361 Rat
• Cellular Locus: Cytoplasm, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, cytoskeleton, microtubule organizing center, centrosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser33 of Human p53 aa 1-50 / 393.
• Isotype: IgG
• Clone: JE41-97
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: HT-29 cell lysate, HT-29 treated with 100ng/mL Nocodazole for 18 hours cell lysate, HT-29 cells treated with 100ng/mL Nocodazole for 18 hours, mouse liver tissue, human breast cancer tissue, human colon cancer tissue, human colon tissue, rat liver tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 53 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes