CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239]

CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239]

• Description: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck.
• Product Name Alternative: CD3 epsilon antibody CD3e antibody CD3e antigen epsilon polypeptide (TiT3 complex) antibody CD3E antigen epsilon polypeptide antibody CD3E antigen, epsilon subunit antibody CD3e molecule epsilon antibody CD3e molecule, epsilon (CD3 TCR complex) antibody CD3e molecule, epsilon (CD3-TCR complex) antibody CD3E_HUMAN antibody IMD18 antibody T cell antigen receptor complex epsilon subunit of T3 antibody T cell surface antigen T3/Leu 4 epsilon chain antibody T cell surface glycoprotein CD3 epsilon chain antibody T-cell surface antigen T3/Leu-4 epsilon chain antibody T-cell surface glycoprotein CD3 epsilon chain antibody T3E antibody TCRE antibody
• Abbreviation: CD3E
• CAS Number: 9000-83-3
• Swiss Prot: P07766 Human
• Cellular Locus: Cell membrane.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within human CD3 epsilon aa 153-203 (Cytoplasmic).
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IP, IF-Cell, IF-Tissue
• Positive Control: Human thymus tissue lysate, Jurkat cell lysate, human tonsil tissue, human spleen tissue, Jurkat.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IHC-P:1:50-1:1;000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 23 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes