PI3 Kinase p110α Recombinant Rabbit Monoclonal Antibody [SJ0186]

PI3 Kinase p110α Recombinant Rabbit Monoclonal Antibody [SJ0186]

• Description: Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns (4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others. Plays a role in the positive regulation of phagocytosis and pinocytosis.
• Product Name Alternative: 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody caPI3K antibody CLOVE antibody CWS5 antibody MCAP antibody MCM antibody MCMTC antibody MGC142161 antibody MGC142163 antibody p110 alpha antibody p110alpha antibody Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody Phosphatidylinositol-4 antibody Phosphoinositide 3 kinase catalytic alpha polypeptide antibody PI3 kinase p110 subunit alpha antibody PI3-kinase subunit alpha antibody PI3K antibody PI3K-alpha antibody PI3KC A antibody PIK3C A antibody Pik3ca antibody PK3CA antibody PK3CA_HUMAN antibody PtdIns 3 kinase p110 antibody PtdIns-3-kinase subunit alpha antibody PtdIns-3-kinase subunit p110-alpha antibody Serine/threonine protein kinase PIK3CA antibody
• Abbreviation: PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA
• CAS Number: 9000-83-3
• Swiss Prot: P42336 Human, P42337 Mouse
• Cellular Locus: Cytosol, plasma membrane, cytoplasm, lamellipodium, membrane, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide within Human PIK3CA aa 1,001-1,050 / 1,068.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IP, IHC-P
• Positive Control: HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, HepG2 cells, Hela cells, MCF-7 cells, human brain tissue, human liver tissue, mouse liver tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IP: 1-2μg/sample ;IHC-P: 1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 124 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes