ERK2 Recombinant Rabbit Monoclonal Antibody [SZ25-01]

• Description:

  Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

• Product Name Alternative:

  ERK 2 antibody ERK antibody ERK-2 antibody ERT1 antibody Extracellular Signal Regulated Kinase 2 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAPK 1 antibody MAPK 2 antibody Mapk1 antibody MAPK2 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody P38 antibody P40 antibody P41 antibody p42-MAPK antibody P42MAPK antibody PRKM1 antibody PRKM2 antibody protein kinase, mitogen-activated, 1 antibody protein kinase, mitogen-activated, 2 antibody protein tyrosine kinase ERK2 antibody

• Abbreviation:

  MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, MAPK1

• Swiss Prot:

  P28482 Human, P63085 Mouse, P63086 Rat

• Cellular Locus:

  Cytoplasm, Nucleus, Cytoskeleton, Membrane, Cell junction.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within Human ERK2 aa 311-360 / 360.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, IHC-Fr

• Positive Control:

  HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, Hela, MCF-7, NIH/3T3, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr: 1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 41 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3