Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Recombinant Rabbit Monoclonal Antibody [SC58-01]

• Description:

  The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

• Product Name Alternative:

  ERK 1 antibody ERK 2 antibody ERK-1 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody HS44KDAP antibody HUMKER1A antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 3 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Microtubule-associated protein 2 kinase antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody Mitogen-activated protein kinase 3 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p41mapk antibody p42-MAPK antibody P42MAPK antibody p44-ERK1 antibody p44-MAPK antibody p44ERK1 antibody p44MAPK antibody PRKM 2 antibody PRKM1 antibody PRKM2 antibody PRKM3 antibody protein tyrosine kinase ERK2 antibody

• Abbreviation:

  MAPK3, MAPK1

• Swiss Prot:

  P27361 Human, P28482 Human, P63085 Mouse, Q63844 Mouse, P21708 Rat, P63086 Rat

• Cellular Locus:

  Cytoplasm, Nucleus.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within Human aa 166-215 / 379.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P, IF-Cell, IF-Tissue, FC, IP

• Positive Control:

  SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, SK-Br-3 whole cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, HeLa treated with 200nM PMA for 30 minutes cell lysate, human thyroid carcinoma tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 41/43 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3