ERK2 Mouse Monoclonal Antibody [A10D2]

• Description:

  Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

• Product Name Alternative:

  ERK 2 antibody ERK-2 antibody ERT1 antibody Extracellular Signal Regulated Kinase 2 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAPK 1 antibody MAPK 2 antibody Mapk1 antibody MAPK2 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody P38 antibody P40 antibody P41 antibody p42-MAPK antibody P42MAPK antibody PRKM1 antibody PRKM2 antibody protein kinase, mitogen-activated, 1 antibody protein kinase, mitogen-activated, 2 antibody protein tyrosine kinase ERK2 antibody

• Abbreviation:

  MAPK1

• Swiss Prot:

  P28482 Human, P63085 Mouse, P63086 Rat

• Cellular Locus:

  Nucleus, spindle, centrosome, cytoplasm, caveola.

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein within human ERK2 aa 200-360.

• Isotype:

  IgG1

• Conjugation:

  Non-conjugated

• Type:

  Mouse monoclonal Antibody

• Applications:

  WB, IHC-P, FC

• Positive Control:

  HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human thyroid tissue, human skin tissue, human breast carcinoma tissue, human pancreas tissue, mouse testis tissue, mouse colon tissue, mouse ovary tissue, K562.

• Concentration:

  2 mg/mL

• Dilution:

  WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

• Purity:

  Protein G affinity purified.

• Form:

  Liquid

• Buffer:

  1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 41 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4