Junctophilin-2 Rabbit Polyclonal Antibody

Junctophilin 2, also known as JPH2, is a protein which in humans is encoded by the JPH2 gene. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic membrane occupation and recognition nexus (MORN) domain that shows specific affinity for the plasma membrane. JPH2 is a member of the junctophilin gene family (the other members of the family are JPH1, JPH3, and JPH4) and is the predominant isoform in cardiac tissue, but is also expressed with JPH1 in skeletal muscle. The JPH2 protein product plays a critical role in maintaining the spacing a geometry of the cardiac dyad - the space between the plasma membrane and sarcoplasmic reticulum. These cardiac dyads also known as junctional membrane complexes or calcium release units are thought to play a key role in calcium induced calcium release by approximating L-type calcium channels on the plasma membrane and ryanodine receptor type 2 on the sarcoplasmic reticulum. JPH2 also contains an evolutionarily conserved nuclear localization signal and a DNA binding domain. During (heart) disease, stress-activated calpain converts the full length JPH2 into fragments. The N-terminal fragment (including nuclear localization signal and DNA binding domain) is translocated to nucleus and regulates gene transcription.

9007-83-4

FLJ40969 antibody JP-2 antibody JP2 antibody Jph2 antibody JPH2_HUMAN antibody Junctophilin 2 antibody Junctophilin type 2 antibody Junctophilin-2 antibody OTTHUMP00000031651 antibody OTTHUMP00000031652 antibody

JP-2

Q9BR39 Human, Q9ET78 Mouse, Q2PS20 Rat

Cell membrane, Sarcoplasmic reticulum membrane, Endoplasmic reticulum membrane; Nucleus.

Synthetic peptide within Human Junctophilin-2 aa 418-467 / 696.

Non-conjugated

Rabbit polyclonal Antibody

WB, IF-Cell, IHC-P, FC

Rat skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue, rat heart tissue, human striated muscle tissue, human heart tissue, mouse skeletal muscle tissue, mouse heart tissue, L6, C2C12.

1 mg/mL

WB: 1:2,000 ;IHC-P: 1:100-1:400 ;FC: 1:50-1:1,000

Immunogen affinity purified.

Liquid

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Predicted band size: 74 kDa

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.