Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein (Monomer, MALS verified)

• Background: The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12V (VVGAVGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVGAVGVGK peptide of the KRASG12V.
• Description: Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein (HLV-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVGAVGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVGAVGVGK).
• Specifications: Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVGAVGVGK peptide
• Host: HEK 293
• Target: HLA-A*1101 | B2M | KRASG12V (VVGAVGVGK)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein (Cat. No. HLV-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.0 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C