PE-Labeled Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Tetramer Protein

PE-Labeled Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Tetramer Protein

• Background: The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12V (VVVGAVGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGAVGVGK peptide of the KRASG12V.
• Description: PE-Labeled Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Tetramer Protein (HLV-HP2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAVGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAVGVGK).
• Specifications: PE-Labeled Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Tetramer Protein is assembled by biotinylated monomer (HLV-H82E7) and PE-labeled streptavidin. Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
• Expression Region: Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAVGVGK peptide
• Host: HEK 293
• Target: HLA-A*1101 & B2M & KRASG12V (VVVGAVGVGK)
• Conjugation: PE Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Bioactivity: Immobilized PE-Labeled Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Tetramer Protein (Cat. No. HLV-HP2H7) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.12-1 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, 1% BSA, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.0 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light