Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Monomer, MALS verified)

• Background:

  The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12V (VVVGAVGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGAVGVGK peptide of the KRASG12V.

• Description:

  Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (HLV-H82E7) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAVGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAVGVGK) .

• Specifications:

  Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 39-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAVGVGK

• Expression Region:

  Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAVGVGK peptide

• Host:

  HEK293

• Target:

  HLA-A*1101 & B2M & KRASG12V (VVVGAVGVGK)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human HLA-A*11:01&B2M&KRASG12V (VVVGAVGVGK) Complex Protein (Cat. No. HLV-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  36.0 kDa and 11.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human