Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

• Background:

  CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

• Description:

  Mouse CD27 Protein, Mouse IgG2a Fc Tag (CD7-M5252) is expressed from human 293 cells (HEK293) . It contains AA Thr 21 - Arg 182 (Accession # P41272-1) .

• Specifications:

  This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P41272-1

• Expression Region:

  Thr 21 - Arg 182

• Host:

  HEK293

• Target:

  CD27

• Conjugation:

  Unconjugated

• Tag:

  C-mFc (mIgG2a)

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  0.1 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  45.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Mouse