Biotinylated Human CD27 / TNFRSF7 Protein, Fc, Avitag™

• Background:

  CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

• Description:

  Biotinylated Human CD27, Fc, Avitag (TN7-H82F6) is expressed from human 293 cells (HEK293) . It contains AA Thr 21 - Ile 192 (Accession # NP_001233) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  NP_001233

• Expression Region:

  Thr 21 - Ile 192

• Host:

  HEK293

• Target:

  CD27

• Conjugation:

  Biotin-labeled

• Tag:

  C-Fc & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human CD27 Ligand, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) with a linear range of 2-78 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  47.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human