Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

• Background: CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
• Description: Mouse CD27 Protein, Mouse IgG2a Fc Tag (CD7-M5252) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Arg 182 (Accession # P41272-1).
• Specifications: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Thr 21 - Arg 182
• Host: HEK 293
• Target: CD27
• Conjugation: Unconjugated
• Tag: C-mFc(mIgG2a)
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 0.1 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL (QC tested).
• Format: Powder
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 45.1 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C