Biotinylated Human Latent TGF-beta 3 / Latent TGFB3 Protein, His, Avitag™

• Background:

  Transforming growth factor beta 3 (TGFB3) is also known as TGF-β3, is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. TGF-beta 3 is similar with TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.

• Description:

  Biotinylated Human Latent TGFB3, His, Avitag (TG3-H82E6) is expressed from human 293 cells (HEK293) . It contains AA Leu 24 - Ser 412 (Accession # P10600-1) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 48.4 kDa. The protein migrates as 15 kDa, 40-45 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P10600-1

• Expression Region:

  Leu 24 - Ser 412

• Host:

  HEK293

• Target:

  TGF-beta 3

• Conjugation:

  Biotin-labeled

• Tag:

  N-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGFB3, His,Avitag (Cat. No. TG3-H82E6) with a linear range of 0.3-20 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  48.4 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  20ug*1

• Species:

  Human