Biotinylated Human latent GDF-8 Protein, His, Avitag™

• Background:

  This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]

• Description:

  Biotinylated Human latent GDF-8 Protein, His, Avitag (GD8-H82Q3) is expressed from human 293 cells (HEK293) . It contains AA Asn 24 - Ser 375 (Accession # O14793-1) .

• Specifications:

  This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 43.7 kDa. The protein migrates as 40 kDa and 53-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  O14793-1

• Expression Region:

  Asn 24 - Ser 375

• Host:

  HEK293

• Target:

  GDF-8

• Conjugation:

  Biotin-labeled

• Tag:

  N-Avi & N-10xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent GDF-8 Protein, His,Avitag (Cat. No. GD8-H82Q3) with a linear range of 0.5-31 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  43.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human