Biotinylated Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein, His, Avitag™&Tag Free

• Background:

  Human Integrin alpha 8 beta 1 Heterodimer Protein consists of ITGA8 and ITGB1. The integrin alpha 8 subunit, isolated by low stringency hybridization, is a novel integrin subunit that associates with beta 1. The recently identified alpha 8 integrin subunit associates with beta 1 and is predominantly expressed in smooth muscle and other contractile cells in adult tissues, and in mesenchymal and neural cells during development. In addition, Integrin alpha 8 beta 1 is a receptor for fibronectin and can promote attachment, cell spreading, and neurite outgrowth on fibronectin.

• Description:

  Biotinylated Human ITGA8&ITGB1 Heterodimer Protein, His, Avitag&Tag Free (IT1-H82Wb) is expressed from human 293 cells (HEK293) . It contains AA Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1) (Accession # P53708-1 (ITGA8) & P05556-1 (ITGB1) ) .

• Specifications:

  Biotinylated Human ITGA8&ITGB1 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 116.4 kDa (ITGA8) and 83.7 kDa (ITGB1) . Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGA8) and 100-115 kDa (ITGB1) respectively due to glycosylation.

• Accession Number:

  P53708-1 (ITGA8) & P05556-1 (ITGB1)

• Expression Region:

  Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 8 beta 1

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA8 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82Wb) with a linear range of 0.078-2.5 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  116.4 kDa (ITGA8) & 83.7 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human