Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His, Avitag™&Tag Free

• Background:

  Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.

• Description:

  Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His, Avitag&Tag Free (IT1-H82Wa) is expressed from human 293 cells (HEK293) . It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1) ) .

• Specifications:

  Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.4 kDa (ITGA5) and 83.7 kDa (ITGB1) . Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 100-115 kDa (ITGA5) and 135-150 kDa (ITGB1) respectively due to glycosylation.

• Accession Number:

  P08648-1 (ITGA5) & P05556-1 (ITGB1)

• Expression Region:

  Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 5 beta 1

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Recombinant Human Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) with a linear range of 1-16 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  112.4 kDa (ITGA5) & 83.7 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human