Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His, Avitag™&Tag Free

• Background:

  Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM) . Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. Integrin alpha 4 beta 1 (Alpha-4/beta-1) is receptors for fibronectin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1.

• Description:

  Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free (IT1-H82W1) is expressed from human 293 cells (HEK293) . It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1) ) .

• Specifications:

  Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) and 83.7 kDa (ITGB1) . Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 52.1 kDa) . Consequently ITGA4 migrates as 65-80 kDa, and ITGB1 as 90-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P13612-1 (ITGA4) & P05556-1 (ITGB1)

• Expression Region:

  Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 4 beta 1

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  113.2 kDa (ITGA4) & 83.7 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human