Mouse anti PSA

CAT: 0579-MUB2020PSize: 0.1 mgDry Ice: NoHazardous: No

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CAT#:0579-MUB2020PSize:0.1 mg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a 33 kDa glycoprotein encoded in Humans by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the Serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders.

CAS Number

9007-83-4

UniProt

P07288

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Clone

2B7

Type

Primary Antibodies

Source

2B7 is a Mouse monoclonal IgG1/k antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with Human PSA.

Applications

ELISA, Flow Cytometry, ICC, IHC (frozen), IHC (paraffin), WB

Field of Research

Cancer

Assay Principle

2B7 is suitable for immunocytochemistry, immuno-histochemistry on frozen and paraffin-embedded tissues, ELISA, Western blotting and flow cytometry. Optimal antibody dilution should be determined by titration.

Form

Each vial contains 100 µL 1 mg/mL purified monoclonal antibody in PBS containing 0,09% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Storage Conditions

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product

UniProtKB · P07288

Prostate-specific antigen

KLK3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P07288
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: KLK3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: PSA
EC number: 3.4.21.77
Processing: Precursor
Secondary accessions: C9JXH3, G3V0H4, G3XAE3, Q15096, Q16272, Q86TG8, Q8IXI4

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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