Biotinylated Human CTGF / CCN2 Protein, His, Avitag™ (recommended for biopanning)

• Background:

  Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.

• Description:

  Biotinylated Human CTGF Protein, His, Avitag (CTF-H82E6) is expressed from human 293 cells (HEK293) . It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.2 kDa. The protein migrates as 40-42 kDa and 43-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q5M8T4-1

• Expression Region:

  Gln 27 - Ala 349

• Host:

  HEK293

• Target:

  CTGF

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human CTGF Protein, His,Avitag (Cat. No. CTF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Potassium glutamate and Arginine, pH7.0

• Additives:

  Trehalose

• Molecular Weight:

  39.2 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human