Human Siglec-10 Protein, Mouse IgG2a Fc Tag, low endotoxin

• Background:

  The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

• Description:

  Human Siglec-10 Protein, Mouse IgG2a Fc Tag (SI0-H525b) is expressed from human 293 cells (HEK293) . It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1) .

• Specifications:

  This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 85.6 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q96LC7-1

• Expression Region:

  Met 17 - Thr 546

• Host:

  HEK293

• Target:

  Siglec-10

• Conjugation:

  Unconjugated

• Tag:

  C-mFc (mIgG2a)

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  0.1 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Siglec-10 Protein, Mouse IgG2a Fc Tag (Cat. No. SI0-H525b) at 5 μg/mL (100 μL/well) can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-20 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  85.6 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human