Siglec-10 Recombinant Protein

• Background:

  The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

• NCBI Gene ID:

  89790

• Swiss Prot:

  Q96LC7

• Accession Number:

  NP_149121.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  84.6 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Sialic acid binding Ig like lectin 10

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Whitney G., et al. 2001, Eur J Biochem., 268 (23), 6083-96.

• Background Reference 02:

  Kivi E., et al. 2009, Blood., 114 (26), 5385-92.

• Background Reference 03:

  Munday J., et al. 2001, Biochem J., 355 (Pt 2), 489-97.

• Other Product Names:

  SIGLEC10, MGC126774, PRO940, Siglec10, SLG2

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3