FITC-Labeled Human Siglec-3 / CD33 Protein, Fc Tag

FITC-Labeled Human Siglec-3 / CD33 Protein, Fc Tag

• Background: Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
• Description: FITC-Labeled Human Siglec-3, Fc Tag (CD3-HF256) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1). It is the FITC labeled form of Human Siglec-3, Fc Tag (CD3-H5257).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Asp 18 - His 259
• Host: HEK 293
• Target: Siglec-3
• Conjugation: FITC Labeled
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Human Siglec-3 Antibody at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-3, Fc Tag (Cat. No. CD3-HF256) with a linear range of 0.313-5 μg/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 52.9 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light