FITC-Labeled Human Siglec-3 / CD33 Protein, Fc Tag

• Background:

  Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

• Description:

  FITC-Labeled Human Siglec-3, Fc Tag (CD3-HF256) is expressed from human 293 cells (HEK293) . It contains AA Asp 18 - His 259 (Accession # AAH28152.1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P20138-1 (R69G)

• Expression Region:

  Asp 18 - His 259

• Host:

  HEK293

• Target:

  Siglec-3

• Conjugation:

  FITC Labeled

• Tag:

  C-Fc

• Source:

  Human

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human Siglec-3 Antibody at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-3, Fc Tag (Cat. No. CD3-HF256) with a linear range of 0.313-5 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  52.9 kDa

• Additionnal Information:

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C, avoid light

• Package Size:

  25ug*1

• Host or Source:

  HEK293

• Species:

  Human

• Protein ID:

  P20138-1 (R69G)

• Preservative:

  Trehalose