FITC-Labeled Human Siglec-2 / CD22 Protein, Fc Tag

FITC-Labeled Human Siglec-2 / CD22 Protein, Fc Tag

• Background: B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD22 mediates B-cell B-cell interactions, and may be involved in the localization of B-cells in lymphoid tissues. Siglec-2 / CD22 binds sialylated glycoproteins, one of which is CD45. Siglec2 / CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
• Description: FITC-Labeled Human Siglec-2, Fc Tag (CD2-HF254) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2, Fc Tag (Cat. No. CD2-H5253).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 101.6 kDa. The protein migrates as 130-150 kDa and >250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Asp 20 - Arg 687
• Host: HEK 293
• Target: Siglec-2
• Conjugation: FITC Labeled
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Monoclonal Anti-Human Siglec-2 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2, Fc Tag (Cat. No. CD2-HF254) with a linear range of 0.1-2.5 μg/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 101.6 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light