APC-Labeled Human Siglec-3 / CD33 Protein, His TagStar Staining

APC-Labeled Human Siglec-3 / CD33 Protein, His TagStar Staining

• Background: Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
• Description: APC-Labeled Human Siglec-3 Protein, His Tag (CD3-HA2H3) is produced via conjugation of APC to Human Siglec-3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Siglec-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.5 kDa.
• Expression Region: Asp 18 - His 259
• Host: HEK 293
• Target: Siglec-3
• Conjugation: APC Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, 0.2% BSA, pH7.4
• Additives: Trehalose
• Molecular Weight: 41.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light