Human IFN-γ ELISA Kit, PRO

CAT: 0716-CEA-C006-96testsSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0716-CEA-C006-96testsSize:96 Tests
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IFN-gamma that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
Description
Human IFN-γ ELISA Kit, PRO is expressed from Human
CAS Number
7732-18-5
Reactivity
Human
Target
IFN-gamma
Applications
The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants. It is suitable for potency assay of CAR-T/NK cell therapy. It has been calibrated against a highly purified human IFN-γ and is evaluated with standard from NIBSC/WHO. Reference Reagent INTERFERON GAMMA (Human, rDNA derived) NIBSC code: 82/587.
Stability
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Format
Solid
Components
This assay kit is used to measure the concentrations of Human Interferon-γ (IFN-γ) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-6 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-6 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
Blue ice
Storage Conditions
2-8°C
Package Size
96tests*1
Species
Human

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