E-64

• Description:

  E-64 (Proteinase inhibitor E 64) is a potent irreversible inhibitor against general cysteine proteases with IC50 of 9 nM for papain.

• Product Name Alternative:

  Proteinase inhibitor E 64

• UNSPSC:

  12352005

• Hazard Statement:

  H372

• Target:

  Autophagy; Bacterial; Cathepsin

• Type:

  Reference compound

• Related Pathways:

  Anti-infection; Autophagy; Metabolic Enzyme/Protease

• Applications:

  COVID-19-immunoregulation

• Field of Research:

  Inflammation/Immunology; Cancer

• Assay Protocol:

  https://www.medchemexpress.com/E-64.html

• Purity:

  99.96

• Solubility:

  DMSO : 62.5 mg/mL (ultrasonic) |H2O : 5 mg/mL (ultrasonic)

• Smiles:

  O=C([C@H]1O[C@@H]1C(N[C@H](C(NCCCCNC(N)=N)=O)CC(C)C)=O)O

• Molecular Formula:

  C15H27N5O5

• Molecular Weight:

  357.41

• Precautions:

  H372

• References & Citations:

  [1]Matsumoto K, et al. Structural basis of inhibition of cysteine proteases by E-64 and its derivatives. Biopolymers. 1999;51 (1) :99-107.|[2]Wadhawan M, et al. Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS One. 2014 Mar 25;9 (3) :e93161.|[3]Baldzizhar R, et al. Anti-serpin antibody-mediated regulation of proteases in autoimmune diabetes. J Biol Chem. 2013 Jan 18;288 (3) :1612-9.|[4]Blass G, et al. Chronic cathepsin inhibition by E-64 in Dahl salt-sensitive rats. Physiol Rep. 2016 Sep;4 (17) . pii: e12950.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C, 3 years (Powder)

• Scientific Category:

  Reference compound1

• Clinical Information:

  No Development Reported

• Isoform:

  Cathepsin K; Cathepsin L

• CAS Number:

  66701-25-5