Rabbit anti-EIF4E Antibody

CAT: 0946-YLD2763-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0946-YLD2763-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rabbit polyclonal antibody to EIF4E
CAS Number
9007-83-4
Synonyms
EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Host
Rabbit
Reactivity
H, M, R, B, Mk, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF4E. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of EIF4E expression in HEK293T (A), NIH3T3 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of EIF4E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1977
Gene ID (Mouse)
13684
Gene ID (Rat)
117045
SwissProt (Human)
P06730
SwissProt (Mouse)
P63073
SwissProt (Rat)
P63074

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