Anti-CD11b / MAC-1 (Microglial Marker)(ITGAM/3339)

Anti-CD11b / MAC-1 (Microglial Marker)(ITGAM/3339)

• Description: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin M2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ITGAM
• Gene ID: 3684
• NCBI Gene ID: 172631
• UniProt: P11215
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa941-1074) of human ITGAM protein (exact sequence is proprietary)
• Target Antigen: CD11b | MAC-1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: ITGAM/3339
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Positive Control: Monocytes & granulocytes. Lymph nodes, spleen and tonsil (IHC).
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 95 kDa & 170 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years