Anti-CD11b / MAC-1 (Microglial Marker)(ITGAM/3337)

Anti-CD11b / MAC-1 (Microglial Marker)(ITGAM/3337)

• Description: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin '2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin aM/'2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ITGAM
• Gene ID: 3684
• NCBI Gene ID: 172631
• UniProt: P11215
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa941-1074) of human ITGAM protein (exact sequence is proprietary)
• Target Antigen: CD11b | MAC-1
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: ITGAM/3337
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Positive Control: Monocytes & granulocytes. Lymph nodes, spleen and tonsil (IHC).
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 95 kDa & 170 kDa
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years