Anti-OCT-2 (POU2F2) (B-Cell Marker) (OCT2/2136)

Anti-OCT-2 (POU2F2) (B-Cell Marker) (OCT2/2136)

• Description: Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. Oct-2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of Oct-2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of Oct-2. Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for Oct-2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; POU domain class 2 transcription factor 2; POU2F2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: POU2F2
• Gene ID: 5452
• NCBI Gene ID: 147381
• UniProt: P09086
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)
• Target Antigen: 44836
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: OCT2/2136
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Immunology, Transcription factors
• Positive Control: Raji or U-937 cells. Spleen or Tonsil.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 60 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years