Anti-CD4, Mouse (T-Cell Marker)(GK1.5), CF640R conjugate

• Description:

  CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally. This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD4 (L3T4); CD4; CD4 Lymphocyte deficiency; included; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene ID:

  12504

• NCBI Gene ID:

  2209

• UniProt:

  P06332

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse

• Immunogen:

  Mouse CTL clone V4

• Target Antigen:

  CD4

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  GK1.5

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published)

• Validated Applications:

  FC, Functional assays

• Positive Control:

  Spleen, Thymus or Lymph Node.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Immunological Rev (1982) 74:29-56. (functional studies) J Immunol (2005) 175: 2357-2365. (Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years