Anti-CD4 (T-Helper/Inducer Cell Marker)(CD4/3026)

• Description:

  The immunogen for this Mab is derived from the extracellular domain of CD4, a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4 /CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD4

• Gene ID:

  920

• NCBI Gene ID:

  631659

• UniProt:

  P01730

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human CD4 protein extracellular domain fragment (around aa 245-392) (exact sequence is proprietary)

• Target Antigen:

  CD4

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CD4/3026

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Positive Control:

  Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4