Anti-Vimentin (Mesenchymal Cell Marker)(VIM/1937R), CF405S conjugate

• Description:

  This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e. g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  VIM

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  VIM

• Gene ID:

  7431

• NCBI Gene ID:

  455493

• UniProt:

  P08670

• Cellular Locus:

  Cytoskeleton

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human vimentin protein

• Target Antigen:

  Vimentin

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  VIM/1937R

• Conjugation:

  CF405S

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Positive Control:

  U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  57-60 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years