Anti-Vimentin (Mesenchymal Cell Marker)(VIM/3736)
CAT:
37-BNUM3736-50
Size:
50 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-Vimentin (Mesenchymal Cell Marker)(VIM/3736)
- Description: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e. g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: VIM
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: VIM
- Gene ID: 7431
- NCBI Gene ID: 455493
- UniProt: P08670
- Cellular Locus: Cytoskeleton
- Host: Mouse
- Species Reactivity: Human
- Immunogen: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
- Target Antigen: Vimentin
- Clonality: Monoclonal
- Isotype: IgG2b κ
- Clone: VIM/3736
- Conjugation: Purified, BSA-free
- Source: Animal
- Applications: IHC, FFPE (verified) | WB (verified)
- Validated Applications: IHC, FFPE, WB
- Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.
- Concentration: 1 mg/mL
- Buffer: PBS, no BSA, no azide
- Molecular Weight: 57-60 kDa
- Shipping Conditions: Room temperature
- Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years