Anti-MMP3 (Marker of Metastasis and Rheumatoid Arthritis)(1B4), Biotin conjugate

• Description:

  The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MMP3; Proteoglycanase; SL1; STMY1; STR1; Stromelysin-1; Transin 1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  MMP3

• Gene ID:

  4314

• NCBI Gene ID:

  375129

• UniProt:

  P08254

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment corresponding to amino acids 317-327 of human MMP3.

• Target Antigen:

  MMP3

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  1B4

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  IHC, FFPE (published) | WB (published)

• Validated Applications:

  IHC, FFPE, WB

• Positive Control:

  A431 cells. Prostate or Lung Carcinoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  57 kDa

• Additionnal Information:

  ELISA: Use at 2-4 ug/mL for coating; Order without BSA; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2006) 12(4) 1184-1191. (IHC, FFPE) Placenta (2010) 31(10): 886-892. (WB)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years