Anti-CD62L (L-Selectin)(CD62L/1588), CF405S conjugate

• Description:

  Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  A.11; AI528707; CD62 antigen ligand; CD62L; gp90-MEL; IgA nephropathy; susceptibility to; included; L Selectin; L-selectin; LAM1; LECAM1; LEU8; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; LSEL; Ly-22; LYAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; Pln homing receptor; PLNHR; SELL; TQ1

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SELL

• Gene ID:

  6402

• NCBI Gene ID:

  728756

• UniProt:

  P14151

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.

• Target Antigen:

  CD62L | L-Selectin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  CD62L/1588

• Conjugation:

  CF405S

• Source:

  Animal

• Positive Control:

  HPBL or Jurkat whole cell lysate.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  65-75 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4