Anti-CD62L (L-Selectin)(CD62L/1588), Biotin conjugate

Anti-CD62L (L-Selectin)(CD62L/1588), Biotin conjugate

• Description: Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: A.11; AI528707; CD62 antigen ligand; CD62L; gp90-MEL; IgA nephropathy; susceptibility to; included; L Selectin; L-selectin; LAM1; LECAM1; LEU8; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; LSEL; Ly-22; LYAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; Pln homing receptor; PLNHR; SELL; TQ1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SELL
• Gene ID: 6402
• NCBI Gene ID: 728756
• UniProt: P14151
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
• Target Antigen: CD62L | L-Selectin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: CD62L/1588
• Conjugation: Biotin
• Source: Animal
• Positive Control: HPBL or Jurkat whole cell lysate.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 65-75 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years