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Recombinant Basic Cytokeratin Antibody

CAT: 0013-GTR18317526Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18317526Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa. mAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
UniProt
Q01546
Reactivity
Human
Immunogen
Recombinant full-length human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Clonality
Recombinant
Clone
RKRTH/8883
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa

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