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Recombinant Basic Cytokeratin Antibody

CAT: 0800-V4282-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V4282-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa. mAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Q01546
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2b κ
Clone
RKRTH/8883
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Basic Cytokeratin antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human skin tissue with recombinant Basic Cytokeratin antibody (clone rKRTH/8883) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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