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Basic Cytokeratin Antibody

CAT: 0013-GTR18572271Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572271Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail.
Reactivity
Human, Mouse, Rat
Immunogen
Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.
Clonality
Monoclonal
Clone
AE3
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein G purified
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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