P Glycoprotein Recombinant Mouse Monoclonal Antibody [A10F2-R]

P Glycoprotein Recombinant Mouse Monoclonal Antibody [A10F2-R]

• Description: P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi, and bacteria, and it likely evolved as a defense mechanism against harmful substances. Substrate enters P-gp either from an opening within the inner leaflet of the membrane or from an opening at the cytoplasmic side of the protein. ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a position to be excreted from the cell. Release of the phosphate (from the original ATP molecule) occurs concurrently with substrate excretion. ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a phosphate molecule resets the protein, so that the process can start again.
• Product Name Alternative: ABC20 antibody ABCB1 antibody ATP binding cassette, sub family B (MDR/TAP), member 1 antibody ATP-binding cassette sub-family B member 1 antibody CD243 antibody CLCS antibody Colchicin sensitivity antibody Doxorubicin resistance antibody GP170 antibody MDR1 antibody MDR1_HUMAN antibody Multidrug resistance 1 antibody Multidrug resistance protein 1 antibody P glycoprotein 1 antibody P gp antibody P-glycoprotein 1 antibody PGY1 antibody
• Abbreviation: ABCB1, MDR1, PGY1
• CAS Number: 9000-83-3
• Swiss Prot: P08183 Human, P06795 Mouse, P43245 Rat
• Cellular Locus: Cell membrane, Apical cell membrane, Cytoplasm.
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human P Glycoprotein aa 935-1,280 / 1,280.
• Isotype: IgG1
• Clone: A10F2-R
• Conjugation: Non-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: IHC-P
• Positive Control: Human colon cancer tissue, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
• Concentration: 1 mg/mL
• Dilution: IHC-P: 1:5,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 142 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes