Canine CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

Canine CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

• Background: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Description: Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (CDD-C52W3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D) (Accession # P27597-1 (CD3E) & XP_536556.1 (CD3D)).
• Specifications: Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa (CD3E) under reducing (R) condition, and 36-45 kDa (CD3D) under non-reducing (NR) condition (SDS-PAGE).
• Expression Region: Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D)
• Host: HEK 293
• Target: CD3E & CD3D
• Conjugation: Unconjugated
• Tag: C-10×His | Native
• Source: Canine
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 16.6 kDa (CD3E) & 13.3 kDa (CD3D)
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C